Alstembio/RetrovirusiPCreProgrammingKit/RF101-免疫在线蚂蚁淘旗下平台

商品详细Alstembio/RetrovirusiPCreProgrammingKit/RF101

Alstembio/RetrovirusiPCreProgrammingKit/RF101

商品编号： RF101

品牌： ALSTEM

市场价： ¥19000.00

美元价： 11400.00

产地：美国(厂家直采)

公司：

产品分类：细胞代谢

公司分类： cellular_metabolism

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

RetrovirusiPSCReprogrammingKit:#RF101

ProductDescription

Humaninducedpluripotentstemcells(iPSCs)canbederivedfromsomaticcellsthroughareprogrammingprocessdrivenbyectopicexpressionofadefinedsetofreprogrammingfactors:Oct4,Sox2,Klf4andc-Myc.ThesehiPSCssharethepropertiesofself-renewalandpluripotencywithhumanEScells,andcanthereforebeusedasarenewablesourceforalldifferentiatedcelltypesofthebody.HumaniPSCscanbegeneratedfrompatientsofvirtuallyanygeneticbackground.Retrovirusesareefficienttoolsfordeliveringheritablegenesintothegenomeofdividingcells.TheVSV-Gpseudotypedretrovirushasawiderangeoftargetsincludingbothmammalianandnon-mammaliancells,andareusuallysilencedinEScells.TheretrovirusiscommonlyusedingeneratingiPSCsbecauseofitshighreprogrammingefficiency.TheHumaniPSCellReprogrammingRetrovirusKitofferssuchopportunitytogenerateiPSCsfromvarioustissuesandcelltypes.

ProductSpecifications
ProductName	RetrovirusiPSCReprogrammingKit
Catalog#	RF101
Content	Retroviruscocktailcontaining:humanOct4retrovirus(cat#RF01O)2vials,20μleachhumanSox2retrovirus(cat#RF01S)2vials,20μleachhumanKlf4retrovirus(cat#RF01K)2vials,20μleachhumanc-Mycretrovirus(cat#RF01M)2vials,20μleachGFPretrovirus(cat#RF01G)1vial,20μleachTransPlusvirustransductionenhancer(cat#V020)1vial,100μl
Shipping	DryIce
StorageandStABIlity	Storeat-80°C.Thisproductisstableupto6monthswhenstoredasdirected.
QualityControl	EachlotofHumaniPSCellReprogrammingRetrovirusKitistestedforsterilityandsuccessfulreprogramming.
RestrictedUse	ForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures.
Protocol	Download

Overview
Procedure

Humaninducedpluripotentstemcells(iPSCs)canbederivedfromsomaticcellsthroughareprogrammingprocessdrivenbyectopicexpressionofadefinedsetofreprogrammingfactors:Oct4,Sox2,Klf4andc-Myc.ThesehiPSCssharethepropertiesofself-renewalandpluripotencywithhumanEScells,andcanthereforebeusedasarenewablesourceforalldifferentiatedcelltypesofthebody.HumaniPSCscanbegeneratedfrompatientsofvirtuallyanygeneticbackground.

Retrovirusesareefficienttoolsfordeliveringheritablegenesintothegenomeofdividingcells.TheVSV-Gpseudotypedretrovirushasawiderangeoftargetsincludingbothmammalianandnon-mammaliancells,andisusuallysilencedinEScells.TheretrovirusiscommonlyusedingeneratingiPSCsbecauseofitshighreprogrammingefficiency.TheHumaniPSCellReprogrammingRetrovirusKitofferssuchopportunitytogenerateiPSCsfromvarioustissuesandcelltypes.

Materialsyouwillneed:

HumanESmedium

DMEM/F12	Invitrogen	10565-042
Pen/strep	Invitrogen	15140-122	1X	1mL
L-Glutamax	Invitrogen	35050-061	2mM	2mL
nonessentialaminoacids	Invitrogen	11140-050	0.1mM	2mL
2-mercaptoethanol	Sigma	M7522	0.1mM(1000x)	0.2mL
knockoutserumreplacement	Invitrogen	10828-028	20%	40mL
bFGF	StemRD	bFGF-050	10ng/mL	0.2mL

Total200mL

品牌介绍

AlstembioiPSC一代服务申请表无风险的一站式服务我们高效的定制iPSC生成服务使您摆脱了繁重的单元重编程过程，因此您可以放松身心并享受自己的研究。我们的iPSC生成服务的主要功能：保证：在极少数情况下无法成功进行编程的情况下，ALSTEM对项目总额具有独特的100％退款政策。这使您可以无风险地试用我们的服务。接受多种供体细胞类型：我们已经成功地从成纤维细胞，PBMC，CD34和LCL生成了iPSC；尖端技术：我们提供无馈线，非集成式重新编程；灵活的重编程方法：我们提供附加型和逆转录病毒iPSC重编程；交付物的多种选择： ALSTEM旨在提供5个来自不同菌落的iPSC小瓶（> 2 x 10 ^ 5个细胞/小瓶）；短期： 2-3个月，取决于重新编程的方法，源细胞和种类；iPSC表征：多能性标志物Oct4和TRA-1-60的AP染色和免疫染色；其他“附加”服务：基因组编辑，畸胎瘤形成，胚状体形成，核型分析和进一步鉴定；