Alstembio/EZQuant&trade；CellQuantifyingKit/CQ10/5000测试
市场价：
¥17900.00
美元价：
10740.00
产品分类：
细胞代谢
公司分类：
cellular_metabolism
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EZQuant™CellQuantifyingKit:#CQ01
(UsecouponcodeEZQ15for15%off1x5mlsize)
ProductDescription
TheEZQuant™CellQuantifyingKitusesWST-8toconvenientlydeterminethenumberofviablecellsincellproliferationandcytotoxicityassays.Thereagentisreducedbythemetabolicactivityoflivecellsandtheamountofformazanproducedisdirectlyproportionaltothenumberoflivingcells.OnebottleofEZQuant™yields500testsandnopremixingisrequired.UsingEZQuant™forcellproliferationassayshasbeenshowntocorrelatewellwiththe[3H]-thymidineincorporationassay.Inaddition,EZQuant™exhibitsahigherdetectionsensitivitycomparedtoothertetrazoliumsalts(e.g.,MTT,XTT,MTS,orWST-1).
Features
|  |
ClicktoseeourRESULTS.
LatestCitations
- Peters,MollyC.,etal."ANovelPolyamine-TargetedTherapyforBRAFMutantMelanomaTumors."MedicalSciences6.1(2018):3.
| ProductSpecifications | |
| ProductName | EZQuant™CellQuantifyingKit |
| Catalog# | CQ01 |
| Size | 500tests(CQ01)/2500tests(CQ05)/5000tests(CQ10) |
| Shipping | RoomTemperature |
| StorageandStABIlity | Storeateitherroomtemperature(stableupto6months),0-5°C(stableupto1year)or-20°C(stableupto2years).Forfrequentuse,storeat0-5°C.Thawingandfreezingrepeatedlywillcauseabackgroundincreasethatwillinterferewiththeassay. |
| QualityControl | Appearance,blank,andsensitivitytestsareperformedforeachlot.Onlylotsthatpassthefollowingcriteriaareoffered:BlankAbsorbance(460nm)≤0.700SensitivityAbsorbance(460nm)≥1.000 |
| RestrictedUse | ForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures. |
| Protocol |  Download |
- CellCountProtocol
- CellProliferation/CytotoxicityProtocol
- InoculatecellsUSPension(100μl/well)ina96-wellplate.Pre-incubatetheplateinahumidifiedincubator(e.g.,at37°C,5%CO2).
- Add10μloftheEZQuant™solutiontoeachwelloftheplate.Becarefulnottointroducebubblestothewells,sincetheyinterferewiththeO.D.reADIng.
- Incubatetheplatefor1-4hoursintheincubator.
- Measuretheabsorbanceat450nmusingamicroplatereader.Tomeasuretheabsorbancelater,add10μlof1%w/vSDSor0.1MHCltoeachwell,covertheplateandstoreitwithprotectionfromlightatroomtemperature.Noabsorbancechangeshouldbeobservedfor24hours.
品牌介绍
AlstembioiPSC一代 服务申请表无风险的一站式服务我们高效的定制iPSC生成服务使您摆脱了繁重的单元重编程过程，因此您可以放松身心并享受自己的研究。我们的iPSC生成服务的主要功能：保证：在极少数情况下无法成功进行编程的情况下，ALSTEM对项目总额具有独特的100％退款政策。这使您可以无风险地试用我们的服务。接受多种供体细胞类型：我们已经成功地从成纤维细胞，PBMC，CD34和LCL生成了iPSC；尖端技术：我们提供无馈线，非集成式重新编程；灵活的重编程方法：我们提供附加型和逆转录病毒iPSC重编程；交付物的多种选择： ALSTEM旨在提供5个来自不同菌落的iPSC小瓶（> 2 x 10 ^ 5个细胞/小瓶）；短期： 2-3个月，取决于重新编程的方法，源细胞和种类；iPSC表征：多能性标志物Oct4和TRA-1-60的AP染色和免疫染色；其他“附加”服务：基因组编辑，畸胎瘤形成，胚状体形成，核型分析和进一步鉴定；
