Alstembio/EZStem™StemCellFreezingMedium/M050
市场价:
¥3300.00
美元价:
1980.00
产品分类:
细胞代谢
公司分类:
cellular_metabolism
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
EZStem™StemCellFreezingMedium:#M050
ProductDescription
EZStem™StemCellFreezingMediumwasdevelopedtomaintainxeno-freeandfeeder-freeconditionsduringcryopreservationwhenculturinghumanembryonicstem(ES)andinducedpluripotentstem(iPS)cells.Itisaready-to-usesolutionforcryopreservationofhumanES/iPScells.HumanpluripotentstemcellspreservedwithEZStem™StemCellFreezingMediumresultsinhighcellviABIlityandrecovery,andexpresstypicalpluripotentMarkersafterthawing.EZStem™StemCellFreezingMediumhasbeentestedonbothhumanESandiPScells.Betterresultswereobtainedincomparisonwithbothserum-containingfreezingmediaandcompetingserum-freeproducts,makingthisanidealproductforcryopreservationofvaluablehumanpluripotentstemcells.
ProductSpecifications | |
ProductName | EZStem™StemCellFreezingMedium |
Catalog# | M050 |
Size | 50ml |
Shipping | Ambienttemperature |
StorageandStability | Storeat-20°C.Thisproductisstableupto6monthswhenstoredasdirected. |
QualityControl | HumanEScellswerefrozenusingEZStem™FreezingMedium,thenrecovered,andAPpositivecolonieswerecounted.Afterrecovery,cellspreservedwithEZStem™FreezingMediumhadaminimumof10%moreAPpositivecoloniesthanotherfreezingmethods. |
RestrictedUse | ForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures. |
Protocol | Download |
- Overview
- Procedure
Thisprotocolcanbeusedforthecryopreservationofhumanembryonicstem(hES)cellsculturedwithfeedercellsorinfeeder-freeconditions.Theproceduredescribesthecryopreservationofcellsculturedinonewellofa6-wellplate.Amountscanbescaledupiffreezingmultiplewells,however,only1mlofcellsUSPensionshouldbealiquottedintoeachcryogenicvial.KeepEZStemFreezingMediumoniceatalltimes.
品牌介绍
AlstembioiPSC一代 服务申请表无风险的一站式服务我们高效的定制iPSC生成服务使您摆脱了繁重的单元重编程过程,因此您可以放松身心并享受自己的研究。我们的iPSC生成服务的主要功能:保证:在极少数情况下无法成功进行编程的情况下,ALSTEM对项目总额具有独特的100%退款政策。这使您可以无风险地试用我们的服务。接受多种供体细胞类型:我们已经成功地从成纤维细胞,PBMC,CD34和LCL生成了iPSC;尖端技术:我们提供无馈线,非集成式重新编程;灵活的重编程方法:我们提供附加型和逆转录病毒iPSC重编程;交付物的多种选择: ALSTEM旨在提供5个来自不同菌落的iPSC小瓶(> 2 x 10 ^ 5个细胞/小瓶);短期: 2-3个月,取决于重新编程的方法,源细胞和种类;iPSC表征:多能性标志物Oct4和TRA-1-60的AP染色和免疫染色;其他“附加”服务:基因组编辑,畸胎瘤形成,胚状体形成,核型分析和进一步鉴定;