Alstembio/Celloticityasayasaykit/CA04/2000试验
市场价：
¥9500.00
美元价：
5700.00
产品分类：
细胞代谢
公司分类：
cellular_metabolism
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EZQuant™CytotoxicityAssayKit:#CA01
ProductDescription
TheEZQuant™CytotoxicityAssayKitisusedtoassesscelldeath.ItmeasuresthelevelofLDHreleasedwhencellsundergostressorinjuryfromchemicalsthataretoxictothecells.ThereagentisreducedbyNADHthatwasoxidizedbytheextracellularLDH.Therefore,theamountofformazanproducedisdirectlyproportionaltothenumberofdeadcells.
PairwithEZQuant™CellQuantifyingKit
TheEZQuant™CellQuantifyingKitconvenientlydeterminesthenumberofviablecellsincellproliferationandcytotoxicityassays.Thisproductisusefulfordrugscreeningandcytotoxicitytestingofchemicals.Meanwhile,theEZQuant™CytotoxicityAssayKitdeterminesthenumberofdamagedcellsandthedegreeofcytotoxicity.Combiningthesetwoproductswillallowresearcherstofullyunderstandthecellhealthconditionsundervariouscytotoxicenvironments.
Features
|
ClicktoseeourRESULTS.
| ProductSpecifications | |
| ProductName | CytotoxicityAssayKit |
| Catalog# | CA01 |
| Size | 500tests(CA01)/2000tests(CA04) |
| Shipping | RoomTemperature |
| StorageandStABIlity | Storeat0-5°C |
| QualityControl | Appearance,blank,andsensitivitytestsareperformedforeachlot.Onlylotsthatpassthefollowingcriteriaareoffered:BlankAbsorbance(460nm)≤0.700SensitivityAbsorbance(460nm)≥1.000 |
| RestrictedUse | ForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures. |
| Protocol |  Download |
- Homogenousassay
- Non-homogenousassay
CellConcentrationOptimization
- Collectcellsandwashthemwiththeassaymedium.PreparecellsUSPensionto5×105cells/mlintheassaymedium.
- Add100μloftheassaymediumtoeachwellofaflat-bottom96-welltissuecultureplate.
- Prepare2-foldserialdilutionofeachwellintriplicatesetofwellsforthehigh-control,low-controlandbackgroundcontrol(mediumonly).Serialdilution:Addthecellsuspension(5×105cells/ml)tothefirstwellandmixbypipetting.Thiswellcontainsthemaximumnumberofcells(2.5×104cells/well).Transfer100μlfromthefirstwelltothenextwell,andmixbypipetting.Repeatthisprocedure.
- Incubatetheplateat37°CforanappropriatetimeinaCO2incubator.Usethesameincubationtimeinthecytotoxicityassay.
- Add10μlofthelysisbuffertoeachwellofthehighcontrol.
- Incubatetheplateat37°Cfor30minutesinaCO2incubator.
- Add100μloftheworkingsolutiontoeachwell.Protecttheplatefromlightandincubateitattheroomtemperaturefor30minutes.
- Add50μlofthestopsolutiontoeachwell.
- Measuretheabsorbanceat490nmbyamicroplatereader.
- Add50μlofcellsuspensiontoeachwellofaflat-bottom96-welltissuecultureplate.Foradherentcells:incubatetheplateat37°CovernightinaCO2incubatortoallowthecellstoadhereandthenreplacetheassaymediumwith50μloffreshassaymedium.
- Add50μlofassaymediumcontainingtestsubstancethatadjustedtothedesiredconcentration.
Backgroundcontrol Testsubstance Lowcontrol Highcontrol Assaymedium 100μl N/A 50μl 50μl Cellsuspension N/A 50μl 50μl 50μl Testsubstanceinculturemedium N/A 50μl N/A N/A Lysisbuffer N/A N/A N/A 10μl - Incubatetheplateat37°CforanappropriatetimeperiodinaCO2incubator.
- Add10μlofthelysisbuffertoeachwellofthehighcontrol.Incubatetheplateat37°Cfor30minutesinaCO2incubator.
- Add100μloftheworkingsolutiontoeachwell.Protecttheplatefromlightandincubateitattheroomtemperaturefor30minutes.
- Add50μlofthestopsolutiontoeachwell.
- Measuretheabsorbanceat490nmbyamicroplatereader.
Calculatetheaverageabsorbancefromeachtriplicatesetofwellsandsubtractthebackgroundcontrolvaluefromeachabsorbanceone.Calculatethepercentcytotoxicitybythefollowingequation:Cytotoxicity(%)=(Testsubstance-Lowcontrol)/(Highcontrol-Lowcontrol)x100
品牌介绍
AlstembioiPSC一代 服务申请表无风险的一站式服务我们高效的定制iPSC生成服务使您摆脱了繁重的单元重编程过程，因此您可以放松身心并享受自己的研究。我们的iPSC生成服务的主要功能：保证：在极少数情况下无法成功进行编程的情况下，ALSTEM对项目总额具有独特的100％退款政策。这使您可以无风险地试用我们的服务。接受多种供体细胞类型：我们已经成功地从成纤维细胞，PBMC，CD34和LCL生成了iPSC；尖端技术：我们提供无馈线，非集成式重新编程；灵活的重编程方法：我们提供附加型和逆转录病毒iPSC重编程；交付物的多种选择： ALSTEM旨在提供5个来自不同菌落的iPSC小瓶（> 2 x 10 ^ 5个细胞/小瓶）；短期： 2-3个月，取决于重新编程的方法，源细胞和种类；iPSC表征：多能性标志物Oct4和TRA-1-60的AP染色和免疫染色；其他“附加”服务：基因组编辑，畸胎瘤形成，胚状体形成，核型分析和进一步鉴定；
