犬骨髓基质干细胞片层制备及向成骨细胞的分化_图文 - 百度文库

来自 : 发布时间：2024-05-17

卜令学;荆恒;陈立强;高振华;李宁毅【期刊名称】《中国组织工程研究》【年(卷),期】2010(014)040【摘要】BACKGROUND:Conventionalmethods,includingtrypsindigestionandcellstransferusingsinglecallsuspension,havemanydrawbacks,whichlimitthedevelopmentofbonetissueengineering.OBJECTIVE:Toculturebonemarrowstromalstemcalls,induceosteoblasticdifferentiation,andpreparecellsheets.METHODS:Caninebonemarrowstromalcallswereisolatedbydensitygradientcentrifugationtechnique,inoculatedintoDMEMmedium,andinducedtodifferentiateintoosteoblasts.Completecallsheetswereharvestedbycallsheetengineeringbasedonthetemperaturechangeoftemperature-responsivemedium.RESULTSANDCONCLUSION:Immediatelyafterinoculation,primarycallswerescatteredonthebottomofcultureflask,presentingatransparentsphericalbodywithagoodrefractivecapacity.At12hours,callsexhibitedalongshuttleshape,reachedcompleteconfluency,andgrewinawhirlpool-likefashion.Afterosteoblasticinduction,themajorityofbonemarrowstromalstemcallsappearedtetragonal,polygonal,andsquamose.At21-28days,roundoroval-shapedcalcifiednodulesformed.Whenthebonemarrowstromalstemcallsinthetemperature-